Engineered Veterinary Vectored Immunotherapy and Vaccines: EVVIVAX

Just open a Bet365 account today and make a deposit http://abonuscode.co.uk Make a deposit of £10-£200 and then enter the 10-digit bonus code

Tumor Reduction Demonstrated In Vivo Using Two Linear DNA Vaccines in Animal Models

Tumor Reduction

STONY BROOK, N.Y., September 19, 2019 – Applied DNA Sciences Inc. (NASDAQ: APDN) (“Applied DNA”), a leader in large-scale PCR-based DNA manufacturing, announced today that LineaRx, Inc. (“LineaRx”), its majority-owned subsidiary focused on next-generation biotherapeutics, and its collaborators Takis/Evvivax have completed pre-clinical animal studies of two vaccine candidates produced with linear DNA. Tests of both vaccines in mouse models demonstrated their ability to eliminate tumors in vivo, and to potentially prevent initial occurrence.

Evvivax S.R.L. designed a synthetic gene sequence to produce immunity in companion animals, directed against the protein telomerase (TERT), which is overexpressed in 85% of all mammalian cancers, whereas Takis S.R.L. designed a neoantigen-based cancer vaccine (TK), as a proof-of-concept of personalized immunology. For the studies, the TERT and TK based vaccines were produced in linear DNA form by LineaRx. Here is a summary of the recently completed studies performed in vivo:

Previous results from Phase 1 of the collaboration’s Joint Development Agreement (JDA) confirmed immunogenicity in mice that were vaccinated with linear DNA against the human protein telomerase. The results reported herein are from the Phase 2 of the JDA using mice implanted with murine colon cancer.
In the prophylactic use of the telomerase vaccine, wherein vaccination is done before addition of tumor cell lines to the mice, transplanted tumors were cleared within 10 days.
In the therapeutic application using either TERT or TK linear DNA vaccine application, cancer cells were introduced and allowed to grow before administration of the vaccine. The successful reduction of total tumor mass was accomplished in conjunction with antibodies that targeted PD-1 and CTLA4. These antibodies, known as Checkpoint Inhibitors, are commonly used in oncology strategies that rely upon immunotherapy. In this setting the tumor cells were reduced significantly within 30 days.
The strategy of the Takis TK neoantigen vaccine uses unique multiple-target antigenic epitopes that have been identified from the tumor of a human patient. The linear DNA can utilize the genes for several of the most effective universal antigens such as TERT, or neoantigens that are more personalized to the patient.

“We know that immune system has a key role in fighting cancer” said Dr. Luigi Aurisicchio, CEO of Takis/Evvivax. “Thanks to a deep knowledge of cancer immunobiology and cutting-edge vaccination technologies we have now the tools to provide new therapies to our pet friends and human patients.”

For more information please see the Press Release from Applied DNA Sciences adnas

 

Just open a Bet365 account today and make a deposit http://abonuscode.co.uk Make a deposit of £10-£200 and then enter the 10-digit bonus code

 University Hospital for Companion Animals

Our CEO/CSO Luigi Aurisicchio flew to Copenaghen to present promising results about electrochemotherapy at the University Hospital for Companion Animals.

Electrochemotherapy (ECT) is an approved and efficacious treatment for local control of cutaneous and subcutaneous tumor nodules, although less effective against distant or inaccessible metastasis.

In addition, preliminary evidences suggest that ECT can activate the immune system. We therefore investigated the synergic effect of ECT and DNA Electro-Gene Transfer (DNA-EGT) in both mice and dogs by means of a clinically validated device for Veterinary electroporation called Vet-ePorator. In particular, we explored the combination of our vaccine Erb-eVax and ECT in dogs with metastatic breast cancer or osteosarcoma, with excellent results in terms of both tumor control and immune response against Tumor Associated antigens.

Our findings provide new data on tumor immunobiology and most importantly improve the efficacy of ECT and may open up novel immunotherapeutic approaches.

We are pleased to share these data that summarize the best of our technology and make a significant impact on patients’ survival and quality of life.

 

Just open a Bet365 account today and make a deposit http://abonuscode.co.uk Make a deposit of £10-£200 and then enter the 10-digit bonus code

 

Flyer A4 Meeting Vitares 2019 Bn

Evvivax is glad to sponsor the first Vitares Annual Congress, which is also the first Italian One Health meeting focusing on Cancer Immunotherapy.

This meeting brings together Basic research, Veterinary and Human Medicine Scientists involved in the field of Immunology, Oncology and Education, aiming at creating networks of collaboration. Internationally renowned scientists from Italy and abroad will provide the State of the Art and present their most recent data in a highly stimulating scientific environment.

Public health initiatives such as One Health demonstrate the growing awareness of the fact that animal and human health are strongly interconnected with each other and with the environment. Zoonotic pathogens and Cancer represent a direct hazard to Veterinary and Human Health”, said Luigi Aurisicchio, CEO/CSO at Takis and president at Vitares.

Looking forward to meeting you in Rome!

Just open a Bet365 account today and make a deposit http://abonuscode.co.uk Make a deposit of £10-£200 and then enter the 10-digit bonus code

 

EVVIVAX conference veterinary vaccinology

Dr. Antonella Conforti represents EVVIVAX at the fifth UK & International Veterinary Vaccinology Network Conference. Topics will range from vaccine commercialisation to antigen discovery, with scientists from all over the world delivering speeches about vaccine research.

EVVIVAX has expertise in genetic cancer vaccines for pets and is committed to developing new therapeutic strategies with strong translational potential. We cannot miss this appointment, where innovative approaches and incoming challenges in veterinary vaccinology will be addressed, with the awareness that fighting animal diseases will also affect men’s health.

This year, we are plenty of ideas to further expand our research activities: we are excited to share our knowledge and look forward opportunities to network and form new collaborations in London!

ABOUT THE VETERINARY VACCINOLOGY NETWORK

EVVIVAX conference veterinary vaccinology2

The Veterinary Vaccinology Network is a multidisciplinary community who aims to address the unmet needs in veterinary vaccinology in the fight against animal diseases and consequently those that have the potential to spread to humans. The network aims to enhance collaborations between scientific researchers, industry, policy makers and regulators to design, develop and deliver safe and effective next-generation vaccines against new and (re)-emerging diseases.

See more at http://www.vetvaccnet.ac.uk/uk-and-international-veterinary-vaccinology-network-conference-2019

Just open a Bet365 account today and make a deposit http://abonuscode.co.uk Make a deposit of £10-£200 and then enter the 10-digit bonus code

Applied DNA Sciences Inc. ("Applied DNA" or the "Company") announced that LineaRx, Inc. ("LineaRx"), its wholly-owned subsidiary focused on next-generation biotherapeutics, has signed a Joint Development Agreement (the "Agreement") with Takis S.R.L. and Evvivax S.R.L. ("Takis/Evvivax"), biotechnology companies focused on the discovery and development of DNA based anti-cancer vaccines for the human and animal markets, respectively.

Under the terms of the Agreement, LineaRx and Takis/Evvivax will jointly develop linear DNA expression vectors for two of Takis/Evvivax's anti-cancer vaccine candidates utilizing LineaRx's linear DNA technology. Linear DNA amplicons carrying the DNA sequences for Takis/Evvivax vaccine candidates will be delivered to preclinical animal models via Takis/Evvivax's proprietary electroporation technology. Antigen-specific immune responses aimed at achieving therapeutic effects will be studied.

The previously announced collaboration between the companies has already shown promise of yielding immunity in mice that were DNA-vaccinated against the human protein telomerase, which is over-expressed in more than 85% of all cancers.

Dr. Luigi Aurisicchio, CEO of Takis/Evvivax stated: "We are excited to co-develop linear DNA expression vectors for our DNA vaccine candidates with LineaRx. Initial data from the use of LineaRx's amplicons in our vaccine candidates is promising. The advantages of linear DNA over plasmids would provide a clear market edge over plasmid-based DNA sources. We look forward to a mutually beneficial collaboration".

"This Agreement serves to validate LineaRx's technology as critical and necessary to the field of biotherapeutics in its ability to deliver potentially powerful approaches to the treatments of chronic diseases," stated Dr. James Hayward, president and CEO of Applied DNA. "Takis/Evvivax are ideal partners of LineaRx given their innovative anti-cancer vaccine candidates for both humans and animals together with their expertise in preclinical animal models."

Dr. Hayward continued, "The use of PCR-produced linear DNA, as opposed to bacterially produced plasmids, is an innovative concept that provides the potential for increased patient safety, ease of manufacture and vaccine logistics, and reduced costs. Our know-how in the fields of bulk linear DNA production and in bioconjugate chemistry enable us to create novel and highly efficient expression vectors."

With their stability at room temperature, low risk of infection or secondary illness, and stability during transportation, DNA vaccines overcome many of the undesirable properties of conventional vaccines. The global DNA based human vaccine market is expected to grow at a CAGR of 55% and reach a value of $2.7 billion by 2019. The global veterinary vaccine market is expected to reach $20.6 billion by 2021, with DNA based animal vaccines gaining rapid market share.

Download PDF Copy

 

Evvivax News